Cloning and expression of a putative metacaspase gene from Schizophyllum commune
نویسندگان
چکیده
منابع مشابه
Functional Cloning and Expression of the Schizophyllum commune Glucuronoyl Esterase Gene and Characterization of the Recombinant Enzyme
The gene encoding Schizophyllum commune glucuronoyl esterase was identified in the scaffold 17 of the genome, containing two introns of 50 bp and 48 bp, with a transcript sequence of 1179 bp. The gene was synthesized and cloned into Pichia pastoris expression vector pGAPZα to achieve constitutive expression and secretion of the recombinant enzyme in soluble active form. The purified protein was...
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The patterns of polypeptides synthesized by two co-isogenic monokaryons and the derived dikaryon of the basidiomycete Schizophyflum commune, growing in surface culture, were compared by two-dimensional PAGE after labelling with [35S]sulphate. Synthesis of most of the polypeptides (400 analysed) was common but at day 3 some 30 polypeptides were synthesized in the dikaryon which were not seen in ...
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HE genetic basis of incompatibility in the higher fungi was described T f o r the first time by KNIEP (1920) using the tetrapolar hymenomycete SchizophyZlum commwe. Subsequent work, in various countries, fully confirmed KNIEP’S statement and extended its validity to numerous other basidiomycetes. This later work, however, though voluminous, added little fundamental new knowledge. I t is only ve...
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ژورنال
عنوان ژورنال: The FASEB Journal
سال: 2008
ISSN: 0892-6638,1530-6860
DOI: 10.1096/fasebj.22.1_supplement.1003.12